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初轨确定算法；单X射线脉冲星探测器；相位增量；二体运动方程；加权最小二乘法 1

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Lightweight design of an electric bus body structure with analytical target cascading

《机械工程前沿（英文）》 2023年 第18卷 第1期 doi: 10.1007/s11465-022-0718-y

摘要： Lightweight designs of new-energy vehicles can reduce energy consumption, thereby improving driving mileage. In this study, a lightweight design of a newly developed multi-material electric bus body structure is examined in combination with analytical target cascading (ATC). By proposing an ATC-based two-level optimization strategy, the original lightweight design problem is decomposed into the system level and three subsystem levels. The system-level optimization model is related to mass minimization with all the structural modal frequency constraints, while each subsystem-level optimization model is related to the sub-structural performance objective with sub-structure mass constraints. To enhance the interaction between two-level systems, each subsystem-level objective is reformulated as a penalty-based function coordinated with the system-level objective. To guarantee the accuracy of the model-based analysis, a finite element model is validated through experimental modal test. A sequential quadratic programming algorithm is used to address the defined optimization problem for effective convergence. Compared with the initial design, the total mass is reduced by 49 kg, and the torsional stiffness is increased by 17.5%. In addition, the obtained design is also validated through strength analysis.

关键词： electric vehicle     body in white (BIW)     lightweight     analytical target cascading (ATC)    

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Loss of monocarboxylate transporter 1 aggravates white matter injury after experimental subarachnoid

《医学前沿（英文）》 2021年 第15卷 第6期   页码 887-902 doi: 10.1007/s11684-021-0879-9

摘要： Monocarboxylic acid transporter 1 (MCT1) maintains axonal function by transferring lactic acid from oligodendrocytes to axons. Subarachnoid hemorrhage (SAH) induces white matter injury, but the involvement of MCT1 is unclear. In this study, the SAH model of adult male Sprague-Dawley rats was used to explore the role of MCT1 in white matter injury after SAH. At 48 h after SAH, oligodendrocyte MCT1 was significantly reduced, and the exogenous overexpression of MCT1 significantly improved white matter integrity and long-term cognitive function. Motor training after SAH significantly increased the number of ITPR2+SOX10+ oligodendrocytes and upregulated the level of MCT1, which was positively correlated with the behavioral ability of rats. In addition, miR-29b and miR-124 levels were significantly increased in SAH rats compared with non-SAH rats. Further intervention experiments showed that miR-29b and miR-124 could negatively regulate the level of MCT1. This study confirmed that the loss of MCT1 may be one of the mechanisms of white matter damage after SAH and may be caused by the negative regulation of miR-29b and miR-124. MCT1 may be involved in the neurological improvement of rehabilitation training after SAH.

关键词： microRNAs     monocarboxylate transporter 1     motor training     subarachnoid hemorrhage     white matter injury    

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Production of a polyclonal antibody to the VP26 nucleocapsid protein of white spot syndrome virus (wssv

Suchera LOYPRASERT-THANANIMIT, Akrapon SALEEDANG, Proespichaya KANATHARANA, Panote THAVARUNGKUL, Wilaiwan CHOTIGEAT

《化学科学与工程前沿（英文）》 2012年 第6卷 第2期   页码 216-223 doi: 10.1007/s11705-012-1289-y

摘要： White spot syndrome virus (WSSV) is a major cause of high mortality in cultured shrimp all over the world. VP26 is one of the structural proteins of WSSV that is assumed to assist in recognizing its host and assists the viral nucleocapsid to move toward the nucleus of the host cell. The objective of this work was to produce a polyclonal antibody against VP26 and use it as a biosensor. The recombinant VP26 protein (rVP26) was produced in (BL21), purified and used for immunizing rabbits to obtain a polyclonal antibody. Western blot analysis confirmed that the antiserum had a specific immunoreactivity to the VP26 of WSSV. This VP26 antiserum was immobilized onto a gold electrode for use as the sensing surface to detect WSSV under a flow injection system. The impedance change in the presence of VP26 was monitored in real time. The sensitivity of the biosensor was in the linear range of 160–160000 copies of WSSV, indicating that it is good and sensitive for analysis of WSSV. The specificity of the biosensor was supported by the observation that no impedance change was detected even at high concentrations when using Yellow Head Virus (YHV). This biosensor may be applied to monitor the amount of WSSV in water during shrimp cultivation.

关键词： recombinant protein     polyclonal antibody     label-free biosensor     impedance     white spot syndrome virus (WSSV)    

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Prevention and treatment of pancreatic fistula after pancreatic body and tail resection: current status

Li Jiang, Deng Ning, Xiaoping Chen

《医学前沿（英文）》 2020年 第14卷 第3期   页码 251-261 doi: 10.1007/s11684-019-0727-3

摘要： Postoperative pancreatic fistula (POPF) is the most common and critical complication after pancreatic body and tail resection. How to effectively reduce the occurrence of pancreatic fistula and conduct timely treatment thereafter is an urgent clinical issue to be solved. Recent research standardized the definition of pancreatic fistula and stressed the correlation between POPF classification and patient prognosis. According to the literature, identification of the risk factors for pancreatic fistula contributed to lowering the rate of the complication. Appropriate management of the pancreatic stump and perioperative treatment are of great significance to reduce the rate of POPF in clinical practice. After the occurrence of POPF, the treatment of choice should be determined according to the classification of the pancreatic fistula. However, despite the progress and promising treatment approaches, POPF remains to be a clinical issue that warrants further studies in the future.

关键词： pancreatic fistula     pancreatic body and tail resection     distal pancreatectomy    

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Laboratory experiment on using non-floating body to generate electrical energy from water waves

Arunachalam AMARKARTHIK, Srinivasan CHANDRASEKARAN, Karuppan SIVAKUMAR, Harender SINHMAR

《能源前沿（英文）》 2012年 第6卷 第4期   页码 361-365 doi: 10.1007/s11708-012-0210-1

摘要： This paper describes an innovative method of using a non-buoyant body to harness ocean waves. All the point absorbers are buoyant in nature and move up due to buoyancy and come down because of gravity. The point absorbers are designed to move along the waves to make the device efficient. These devices face excessive stress during the rough weather on account of the extreme motion of waves and cause the total device failure. The present study shows that using a non-buoyant body for conventional point absorber principle is much efficient and safer than any other device proposed till today. A small scale wave energy converter with non-buoyant body was designed, fabricated and tested in small scale wave maker. An electrical generator was coupled with the device to generate electrical energy from harnessed waves. The generator was electrically loaded and the generated power was measured. It was found from the experiments that the proposed device showed a significant improvement in electricity generation and safety during extreme conditions. In addition to the electricity generation, the characteristics of the device were also studied by using various wave and device parameters.

关键词： ocean wave energy     point absorbers     heaving body     non-floating object     heave response ratio     electrical energy generation    

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A microarray study of altered gene expression during melanoblasts migration in normal pigmented White

Yulin LI,Deping HAN,Junying LI,Dawn KOLTES,Xuemei DENG

《农业科学与工程前沿（英文）》 2014年 第1卷 第4期   页码 299-306 doi: 10.15302/J-FASE-2014040

摘要： Melanoblasts originating from neural crest cells can migrate through the mesenchyme of the developed embryo and give rise to melanocytes. Unlike the melanocytes that are confined to the integument in other vertebrates, melanocytes in Silky Fowl can reach the ventral regions of the embryos owing to differences in gene expression in the process of melanoblasts migration. In this study, we used microarray profiling to identify differences in gene expression between White Leghorn and Silky Fowl. Differential expression of 2517 microarray probes ( <0.01, Fold Change>2) was observed in Silky Fowl compared to White Leghorn. After filtration by cluster analysis, functional annotation and pathway analysis, eight differentially expressed genes were identified to be closely related to the development of melanocytes. Moreover, differences in expression of immune genes were also detected between Silky Fowl and White Leghorn. The differentially expressed genes associated with melanocyte development were verified by q-PCR, and results were highly consistent with the microarray data. The genes with significantly altered expression involved in melanoblast migration and development suggested that different microenvironments resulted in the abnormal melanoblast migration in Silky Fowl, although there were no big differences in melanoblast development between these two breeds. The candidate genes discovered in this study are beneficial to understand the molecular mechanism of hyperpigmentation in Silky Fowl.

关键词： Silky Fowl     White Leghorn     melanoblast migration     gene expression    

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Yesterday, Today and Future of the Engineering Management Body of Knowledge

Hiral Shah,Walter Nowocin

《工程管理前沿（英文）》 2015年 第2卷 第1期   页码 60-63 doi: 10.15302/J-FEM-2015009

摘要： Young engineers are often promoted to leadership roles such as a team leader or a manager within 3 to 6 years of graduating from an undergraduate degree in a technical area. To be competent and successful in their leadership role engineers need to update their engineering management knowledge and learn new skills. The purpose of engineering management body of knowledge (EMBOK) is to delineate the knowledge, skills, and competency areas that are applicable to the engineering management discipline. It basically answers the question, “What does it take to be an effective engineering manager?” The EMBOK is based on a validated study and can be used for curriculum development, certification exams, and professional development programs. This paper describes the 11 domains that are included in the EMBOK and discuss the future publication of the EMBOK Guide. American Society for Engineering Management (ASEM) offers two certification exams based on the EMBOK, Associate Engineering Manager (AEM) and Professional Engineering Manager (PEM).These certification exams can help an individual in the engineering management profession to show that they have kept up in the field and possess the necessary skills and competency to practice in the discipline. A detailed description of this certification program is included in this paper. The future of EMBOK and the certification program is also discussed.

关键词： body of knowledge     engineering management     certification exam     engineering manager     competency areas    

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control of structural responses using an optimal passive tuned mass damper under stationary Gaussian white

Min-Ho CHEY, Jae-Ung KIM

《结构与土木工程前沿（英文）》 2012年 第6卷 第3期   页码 267-280 doi: 10.1007/s11709-012-0170-x

摘要： In this study, the structural control strategy utilizing a passive tuned mass damper (TMD) system as a seismic damping device is outlined, highlighting the parametric optimization approach for displacement and acceleration control. The theory of stationary random processes and complex frequency response functions are explained and adopted. For the vibration control of an undamped structure, the optimal parameters of a TMD, such as the optimal tuning frequency and optimal damping ratio, to stationary Gaussian white noise acceleration are investigated by using a parametric optimization procedure. For damped structures, a numerical searching technique is used to obtain the optimal parameters of the TMD, and then the explicit formulae for these optimal parameters are derived through a sequence of curve-fitting schemes. Using these specified optimal parameters, several different controlled responses are examined, and then the displacement and acceleration based control effectiveness indices of the TMD are examined from the view point of RMS values. From the viewpoint of the RMS values of displacement and acceleration, the optimal TMDs adopted in this study shows clear performance improvements for the simplified model examined, and this means that the effective optimization of the TMD has a good potential as a customized target response-based structural strategy.

关键词： tuned mass damper     parametric optimization     passive control     white noise     earthquake excitation    

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reduces IgE binding ability of allergenic egg white proteins

Sen LI, Marina OFFENGENDEN, Michael G. GÄNZLE, Jianping WU

《农业科学与工程前沿（英文）》 2018年 第5卷 第3期   页码 373-381 doi: 10.15302/J-FASE-2018210

摘要：

Egg white proteins are one of the major allergens. The objective of this study was to investigate the effect of Aspergillus oryzae cultivation on IgE binding ability of egg white proteins. Effect of A. oryzae on egg white proteins was determined using ninhydrin method, SDS-PAGE, ELISA, fluorescence FITC labeling, MALDI-TOF-MS and LC-MS/MS analysis. Adding mycelium of A. oryzae ATCC 1011 and 16868 substantially reduced the IgE binding ability of acidified egg white after 24 h incubation. The binding capacity of egg white proteins to IgE in plasma from four egg allergy patients was almost completely lost after incubation with mycelium of ATCC 16868. Results from SDS-PAGE, free amino acid analysis, MALDI-TOF-MS and LC-MS/MS indicated that there was no substantial protein degradation during incubation. Therefore, the reduction of IgE binding ability of egg white proteins during A. oryzae treatment was probably due to a loss of ~1700 Da mass including a fragment of the ovomucoid N terminus.

关键词： Aspergillus oryzae     egg allergy     egg white proteins     IgE-binding ability     ovomucoid    

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创建新医学的思考

马蔼乃,赵少奎

《中国工程科学》 2007年 第9卷 第10期   页码 19-23

摘要：

西医以实证科学为基础，以分解、还原为主要方法，分工越来越精细，获得了许多医学上的成就，也治愈了许多疾病，但是强调治病，对人的因素重视不够，在维护整个人体健康方面遇到了某些困惑。 中医把人看成“天、地、人”三位一体的整体，从辨证恒动的观点出发，以实践为基础，实施综合、整体治疗，也获得许多医学上的成就，治愈了许多疾病，但是主要凭感觉，取象比类等方法诊治病人，缺乏实证科学的基础。 半个多世纪以来，提倡中西医结合，有一定成效。在钱学森系统科学思想的指导下，依照“突破还原论，发展整体论，达到两者辩证统一的系统论”的哲学理念，提出了中西医的结合点——人体生态系统。人体生态系统是以人体系统为中心与人体之外的环境构成一个整体的复杂系统，针对“人”是一个开放的复杂巨系统，运用综合集成创新的思路，提出了创建新医学——人体生态医学的构想。

关键词： 复杂性科学     开放的复杂巨系统     人体生态系统     人体信息系统     人体生态医学    

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Sodium butyrate activates HMGCS2 to promote ketone body production through SIRT5-mediated desuccinylation

《医学前沿（英文）》 2023年 第17卷 第2期   页码 339-351 doi: 10.1007/s11684-022-0943-0

摘要： Ketone bodies have beneficial metabolic activities, and the induction of plasma ketone bodies is a health promotion strategy. Dietary supplementation of sodium butyrate (SB) is an effective approach in the induction of plasma ketone bodies. However, the cellular and molecular mechanisms are unknown. In this study, SB was found to enhance the catalytic activity of 3-hydroxy-3-methylglutaryl-CoA synthase 2 (HMGCS2), a rate-limiting enzyme in ketogenesis, to promote ketone body production in hepatocytes. SB administrated by gavage or intraperitoneal injection significantly induced blood β-hydroxybutyrate (BHB) in mice. BHB production was induced in the primary hepatocytes by SB. Protein succinylation was altered by SB in the liver tissues with down-regulation in 58 proteins and up-regulation in 26 proteins in the proteomics analysis. However, the alteration was mostly observed in mitochondrial proteins with 41% down- and 65% up-regulation, respectively. Succinylation status of HMGCS2 protein was altered by a reduction at two sites (K221 and K358) without a change in the protein level. The SB effect was significantly reduced by a SIRT5 inhibitor and in Sirt5-KO mice. The data suggests that SB activated HMGCS2 through SIRT5-mediated desuccinylation for ketone body production by the liver. The effect was not associated with an elevation in NAD+/NADH ratio according to our metabolomics analysis. The data provide a novel molecular mechanism for SB activity in the induction of ketone body production.

关键词： sodium butyrate     succinylation     HMGCS2     ketogenesis     SIRT5    

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Water quality monitoring in a slightly-polluted inland water body through remote sensing - Case study

HE Weiqi, LIU Xuehua, CHEN Jining, CHEN Shan

《环境科学与工程前沿（英文）》 2008年 第2卷 第2期   页码 163-171 doi: 10.1007/s11783-008-0027-7

摘要： This study focused on the water quality of the Guanting Reservoir, a possible auxiliary drinking water source for Beijing. Through a remote sensing (RS) approach and using Landsat 5 Thematic Mapper (TM) data, water quality retrieval models were established and analyzed for eight common water quality variables, including algae content, turbidity, and concentrations of chemical oxygen demand, total nitrogen, ammonia nitrogen, nitrate nitrogen, total phosphorus, and dissolved phosphorus. The results show that there exists a statistically significant correlation between each water quality variable and remote sensing data in a slightly-polluted inland water body with fairly weak spectral radiation. With an appropriate method of sampling pixel digital numbers and multiple regression algorithms, retrieval of the algae content, turbidity, and nitrate nitrogen concentration was achieved within 10% mean relative error, concentrations of total nitrogen and dissolved phosphorus within 20%, and concentrations of ammonia nitrogen and total phosphorus within 30%. On the other hand, no effective retrieval method for chemical oxygen demand was found. These accuracies were acceptable for the practical application of routine monitoring and early warning on water quality safety with the support of precise traditional monitoring. The results show that performing the most traditional routine monitoring of water quality by RS in relatively clean inland water bodies is possible and effective.
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Influence of glucose feeding on the ligninolytic enzyme production of the white-rot fungus Phanerochaete

ZHOU Xiaoyan, WEN Xianghua, FENG Yan

《环境科学与工程前沿（英文）》 2007年 第1卷 第1期   页码 89-94 doi: 10.1007/s11783-007-0017-1

摘要： The present work studied the influence of glucose feeding on the ligninolytic enzyme production of in a nitrogen-limited (C/N ratio is 56/8.8 mmol/L) medium. Several sets of shaking flask experiments were conducted. The results showed that 2 g/L glucose feeding on the first day of the culture (24 h after the inoculation) stimulated both fungal biomass growth and enzyme production. The manganese peroxidase (MnP) activity was 2.5 times greater than that produced in cultures without glucose feeding. Furthermore, the glucose feeding mode in fed-batch culture was also investigated. Compared to cultures with glucose feeding every 48 h, cultures with glucose feeding of 1.5 g/L (final concentration) every 24 h produced more enzymes. The peak and total yield of MnP activity were 2.7 and 3 times greater compared to the contrast culture, respectively, and the enzyme was kept stable for 4 days with an activity of over 200 U/L.

关键词： enzyme production     fed-batch culture     nitrogen-limited     inoculation     mmol/L    

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Numerical simulation of multi-body floating piers to investigate pontoon stability

Mostafa Shahrabi, Khosrow Bargi

《结构与土木工程前沿（英文）》 2013年 第7卷 第3期   页码 325-331 doi: 10.1007/s11709-013-0209-7

摘要： The objective of this study is to develop a procedure to analyze the motions of a floating pier comprised of several pontoons that are modeled as rigid bodies and connected to each other by flexible and rigid connectors. Recently, the use offloating piers has increased because of their advantages, such as faster and higher-quality construction, seismic force isolation for a full-scale mooring system, low dependence on local soil conditions and tides, ability to relocate or reconfigure the pier modules during the operation period and 75-100 years of repair-free service. A floating pier consists of a pier, access bridge, mooring system and fender system, each of which comes in many variations to suit different usages and construction considerations. The typical loads used in the design of these piers are dead loads, live loads, mooring loads, fender loads and environmental loads induced by wind, currents and waves. For numerical simulation, three types of piers are used: passenger piers, light-cargo piers and semi-heavy-cargo piers. The selected piers consist of several large pontoons joined by pivots and have a pile-based mooring system. These piers are modeled by SAP2000software as two-dimensional frames that are linked together. As the first step, each type of pier is subjected to loading, and its general behavior is assessed. According to this behavior, the major load combinations are described for the design of piers and analyzed to determine the behavior of the modules. Lastly, according to the analysis results and the safe use and stability considerations, such as the maximum draft and longitudinal gradient, the dimensions of each module in each pier type are presented.

关键词： coastal structures     numerical simulation     floating pier     rigid pontoons     stability    

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White blood cell count and the incidence of hyperuricemia: insights from a community-based study

Jian Liu, Pingyan Shen, Xiaobo Ma, Xialian Yu, Liyan Ni, Xu Hao, Weiming Wang, Nan Chen

《医学前沿（英文）》 2019年 第13卷 第6期   页码 741-746 doi: 10.1007/s11684-017-0579-7

摘要： Hyperuricemia (HUA) is a risk factor for chronic kidney disease (CKD). The relationship between HUA and white blood cell (WBC) count remains unknown. A sampling survey for CKD was conducted in Sanlin community in 2012 and 2014. CKD was defined as proteinuria in at least the microalbuminuric stage or an estimated GFR of 60 mL/(min·1.73 m ). HUA was defined as serum uric acid>420 µmol/L in men and>360 µmol/L in women. This study included 1024 participants. The prevalence of HUA was 17.77%. Patients with HUA were more likely to have higher levels of WBC count, which was positively associated with HUA prevalence. This association was also observed in participants without CKD, diabetes mellitus, hyperlipidemia, or obesity. Multivariate logistic regression analysis showed that WBC count was independently associated with the risk for HUA in male and female participants. Compared with participants without HUA, inflammatory factors such as high-sensitivity C-reactive protein, tumor necrosis factor-α and interleukin 6 increased in participants with HUA. Hence, WBC count is positively associated with HUA, and this association is independent of conventional risk factors for CKD.

关键词： white blood cell count     hyperuricemia     chronic kidney disease     inflammation    

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标题 作者 时间 类型 操作

Lightweight design of an electric bus body structure with analytical target cascading

期刊论文

Loss of monocarboxylate transporter 1 aggravates white matter injury after experimental subarachnoid

期刊论文

Production of a polyclonal antibody to the VP26 nucleocapsid protein of white spot syndrome virus (wssv

Suchera LOYPRASERT-THANANIMIT, Akrapon SALEEDANG, Proespichaya KANATHARANA, Panote THAVARUNGKUL, Wilaiwan CHOTIGEAT

期刊论文

Prevention and treatment of pancreatic fistula after pancreatic body and tail resection: current status

Li Jiang, Deng Ning, Xiaoping Chen

期刊论文

Laboratory experiment on using non-floating body to generate electrical energy from water waves

Arunachalam AMARKARTHIK, Srinivasan CHANDRASEKARAN, Karuppan SIVAKUMAR, Harender SINHMAR

期刊论文

A microarray study of altered gene expression during melanoblasts migration in normal pigmented White

Yulin LI,Deping HAN,Junying LI,Dawn KOLTES,Xuemei DENG

期刊论文

Yesterday, Today and Future of the Engineering Management Body of Knowledge

Hiral Shah,Walter Nowocin

期刊论文

control of structural responses using an optimal passive tuned mass damper under stationary Gaussian white

Min-Ho CHEY, Jae-Ung KIM

期刊论文

reduces IgE binding ability of allergenic egg white proteins

Sen LI, Marina OFFENGENDEN, Michael G. GÄNZLE, Jianping WU

期刊论文

创建新医学的思考

马蔼乃,赵少奎

期刊论文

Sodium butyrate activates HMGCS2 to promote ketone body production through SIRT5-mediated desuccinylation

期刊论文

Water quality monitoring in a slightly-polluted inland water body through remote sensing - Case study

HE Weiqi, LIU Xuehua, CHEN Jining, CHEN Shan

期刊论文

Influence of glucose feeding on the ligninolytic enzyme production of the white-rot fungus Phanerochaete

ZHOU Xiaoyan, WEN Xianghua, FENG Yan

期刊论文

Numerical simulation of multi-body floating piers to investigate pontoon stability

Mostafa Shahrabi, Khosrow Bargi

期刊论文

White blood cell count and the incidence of hyperuricemia: insights from a community-based study

Jian Liu, Pingyan Shen, Xiaobo Ma, Xialian Yu, Liyan Ni, Xu Hao, Weiming Wang, Nan Chen

期刊论文